Deliverable D6.6 includes a detailed planning for the third and final project period of VIRTIGATION’s DEC strategy, as well as all planned DEC tools, materials and activities.

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Deliverable D6.5 includes a summary of the dissemination & communication tools, materials and activities produced in the second project period of VIRTIGATION.

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Deliverable D7.5 includes a summary of all consortium meetings minutes carried out during the second
project period of VIRTIGATION.

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Deliverable 5.5 describes the experimental tests realised in semi-field and in field environment to screen a selection of
plant extracts with insecticidal effects, developed in the frame of the project, for: i) their efficacy on whitefly vectors, especially Bemisia spp.gr. tabaci; ii) their selectivity on natural enemies (both parasitoids and predators), and iii) their side effects on pollinator insects.

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Deliverable 5.2 is exploring the possibility to develop a cross-protection strategy against ToBRFV for control of the virus in tomato crop. In order to optimize the chance of finding a suitable cross-protection candidate isolate, different research strategies were applied, including screening of naturally occurring ToBRFV isolates, as well as creation of attenuated isolates.

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Deliverable 4.3 investigated the competence of different whitefly species transmitting ToLCNDV, as well as the alternative host plants for ToLCNDV and ToBRFV.

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Deliverable 4.2 investigated the possibilities for the long-distance dispersal of viruses via seed. We focused our work on the two fast spreading viruses in Europe: the tomato leaf curl New Delhi virus (ToLCNDV) and tomato brown rugose fruit virus (ToBRFV). We investigated both ‘seed-borne’ and ‘seed-transmitted’ nature of both viruses.

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Deliverable 3.2 developed near-isogenic tomato lines (NIL) carrying all possible combinations of the four known TYLCV-resistant loci, Ty-2 through ty-5, in the same genetic background. The NIL’s
were inoculated with either TYLCV alone, or with TYLCV and betasatellite, and tested for their
resistance level. Indeed, it was found that tomato lines expressing a combination of TYLCV-resistance genes exhibited a very high level of resistance to both TYLCV and betasatellite.

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Deliverable 2.4 outlines the changes made to the Genome Detective Platform to accommodate the collection and analysis of plant virus samples, including metagenomic viral sequences and epidemiological metadata annotations, for the analysis of plant virus outbreaks.

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Deliverable 2.3 compiles the protocols for full length genome sequencing of circular DNA and linear RNA viruses designed to study virus populations, their diversity, geographical distribution and evolution.

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Deliverable 1.3 presents a comprehensive report of the stakeholder network of the VIRTIGATION project gathered so far in regards of the multi-actor approach, and further includes proposed actions and guidelines to enhance the stakeholders participation. It builds on the initial internal guidelines produced at the onset of the project (cf. D1.1).

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Deliverable 1.4 compiles 14 practice abstracts of the results and knowledge generated during the first period (M1-M18) of the VIRTIGATION project. It also presents the results of co-creation activities performed with the help of National Knowledge Brokers in the project’s focus countries. 

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Deliverable 7.4 provides a major update of the initial DMP (cf. D7.2) at the mid-term of VIRTIGATION’s duration, and now also includes a summarised description of the project’s generated, as well as collected and processed research datasets.

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Deliverable 6.4 provides a first report on the actual exploitable results attained up till now at mid-term of the VIRTIGATION project, and further outlines the measures taken so far by result owners to ensure the (post-)project use of their results.

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Deliverable 6.3 provides an update at mid-way in project implementation on the initial DEC plan (cf D6.1).

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Deliverable 7.3 provides a summary of all consortium meeting minutes and the established activity plans for the following period P2.

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Deliverable 6.2 provides a report summarising analyses of all thus far implemented communication and dissemination materials, tools and channels.

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Deliverable 3.1 aims at finding ToBRFV resistance in tomato wild relatives and to introgress them into tomato cultivars. 

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Deliverable 5.1 aims at finding plant extracts with insecticidal effect against the whitefly vector (Bemisia tabaci, MED-type). For this purpose, 12 different candidate plant extracts were tested against various developmental stages of whitefly under controlled laboratory conditions. All experiments were conducted on tomato host (cv. Moneymaker).

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The sample collection deliverable D2.2 includes a list of virus infected plant samples collected in participating countries that will be used to develop protocols for full length viral genome sequencing, study virus populations, their diversity, geographical distribution and evolution.

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The list of identified actors in the VIRTIGATION network deliverable D1.2 includes a categorized list of stakeholders in the project’s multi-actor network, compiled by National Knowledge Brokers and national partners.

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The sample descriptor list deliverable D2.1 includes the list of biological descriptors that will be used to determine the origin, negative impact and dissemination of plant viruses.  

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The initial data management plan deliverable D7.2 describes the project’s data management strategy in compliance with its participation in the EU Horizon 2020 Open Research Data Pilot. It also provides naming and metadata guidelines.  

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The project manual deliverable D7.1 defines the VIRTIGATION consortium operating procedures and summarizes all the required knowledge and best practices for the effective management of the project. 

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The initial internal guidelines for multi-actor approach and co-creation deliverable D1.1 guides the VIRTIGATION consortium through the multi-actor approach and co-creation process implemented in the project. It also identifies the most relevant actors and stakeholders for the VIRTIGATION network. 

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